Common antigenicity between Japanese cedar (Cryptomeria japonica) pollen and Japanese cypress (Chamaecyparis obtusa) pollen, I. H‐2 complex affects cross responsiveness to Cry j 1 and Cha o 1 at the T‐ and B‐cell level in mice

摘要

Common antigenicity among two purified Japanese cedar pollen allergens (Cry j 1 and Cry j 2) and one Japanese cypress pollen allergen (Cha o 1) was explored at the T‐cell and B‐cell level in mice of different H‐2 haplotypes. Cry j 2 did not show any common antigenicity with Cry j 1 or Cha o 1. B10.S (H‐2S) mice immunized with Cry j 1 or Cha o 1 generated T cells and antibodies reactive to both antigens, indicating the common antigenicity of these antigens. C57BL/6 (H‐2b) mice were non‐responders to Cry j 1. BALB/c (H‐2d) mice immunized with Cry j 1 or Cha o 1 and C57BL/6 mice immunized with Cha o 1 generated T cells that were only reactive with the respective immunogen, but produced antibody reactive to both Cry j 1 and Cha o 1, indicating that Cry j 1 and Cha o 1 share their B‐cell epitope but not their T‐cell epitope. This finding may provide a clue for the clarification of the T‐cell and B‐cell epitopes of Cry j 1 and Cha o 1, even though the data are influenced by H‐2 complex restriction in mice. Considering that H‐2 complex restriction affects cross responsiveness to Cry j 1 and Cha o 1 at the T‐ and B‐cell level in mice, we assessed the possible situation in humans exposed sequentially to Japanese cedar pollen and Japanese cypress pollen.